Last data update: 24 January 2024 16:39 CET
Host name: G4 ROSALUC mouse ES cells (LMBP 10507CB)
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| Source: | Mouse |
| Type: | Embryonic stem (ES) cells |
| Cell line designation: | G4 ROSALUC B12 |
| Other designation: | G4 ROSALUC ES |
| Accession number: | LMBP 10507CB |
| Depositor: | Dr T. Hochepied1 2, Dr L. Haenebalcke1 2 and Prof. Dr J. Haigh1 2 1 Inflammation Research Center, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | • 500 ml bottle Knockout DMEM (Invitrogen, cat. no. 10829-018) • 90 ml serum • 6 ml penicillin/streptomycin 100X • 6 ml beta-mercaptoethanol 100X • 6 ml non-essential amino acids 100X • 6 ml glutamine 100X • recombinant mouse LIF 2000 units/ml |
| Selection marker: | puromycin (1 µg/ml) |
| Temperature: | 37°C |
| Cultivation remarks: | Recovery: startup in a 6-well with inactivated feeders after washing. |
| Genotype: | - |
| Phenotype: | - |
| Properties: | - |
| Further information: | - |
| Original reference: | Haenebalcke et al., Stem Cell Rev. 6 (2013), 774-785 [PMID: 23877658] |
| Restricted use: | - VIB/MSH/BCCM MTA - Commercial, for-profit entities require a use license from VIB / MT SINAI prior to the release of the material. |
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Refer in your Materials and Methods: |
G4 ROSALUC mouse ES cells (LMBP 10507CB) is available at BCCM/GeneCorner. It was deposited by Dr T. Hochepied, Dr L. Haenebalcke and Prof. Dr J. Haigh and was published in Haenebalcke et al., 2013. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.